| ProMedica Health Systems | BioHealth | 14,465 | NW Ohio |
| Mercy Health | BioHealth | 8,827 | Lucas |
| University of Toledo | Higher Education | 6,662 | Lucas |
| Whirlpool Corporation | Advanced Manufacturing | 6,182 | NW Ohio |
| FCA US LLC | Automotive | 6,159 | NW Ohio |
| Bowling Green State University | Higher Education | 3,399 | Wood |
| Sauder | Advanced Manufacturing | 2,850 | Fulton |
| St. Rita's Medical Center | BioHealth | 2,492 | Allen |
| Marathon Petroleum Co., LLC | Automotive | 2,200 | Hancock |
| Cooper Tire & Rubber Co. | Advanced Manufacturing | 2,000 | Hancock |
| Magna International | Automotive | 2,000 | NW Ohio |
| General Motors Powertrain | Automotive | 1,971 | Lucas |
| Hearthside Food Solutions | Food Sciences & Agriculture | 1,900 | Hancock |
| HCR ManorCare, Inc. | BioHealth | 1,845 | Lucas |
| Dana Incorporated | Automotive | 1,571 | NW Ohio |
| Campbell Soup Supply Company | Food Sciences & Agriculture | 1,558 | Henry |
| The Anderson's, Inc. | Food Sciences & Agriculture | 1,545 | Lucas |
| Toledo Molding & Die | Automotive | 1,480 | NW Ohio |
| Avita Health Systems | BioHealth | 1,402 | Crawford |
| United Parcel Service | Logistics & Distribution | 1,400 | Lucas |
| Libbey, Inc. | Advanced Manufacturing | 1,300 | Lucas |
| Johns Manville Corporation | Aerospace and Aviation | 1,284 | NW Ohio |
| Owens Corning | Advanced Manufacturing | 1,237 | Lucas |
| Ford Motor Company | Advanced Manufacturing | 1,200 | Allen |
| GM Powertrain | Automotive | 1,102 | Defiance |
| Owens Community College | Higher Education | 1,064 | Wood |
| Toledo Edison/A FirstEnergy Co. | Shale Energy & Petrochemicals | 1,060 | NW Ohio |
| International Automotive Component, Inc. | Automotive | 1,030 | NW Ohio |
| Home Depot Distribution | Logistics & Distribution | 1,025 | NW Ohio |
| Nissin Brake Ohio Inc. | Automotive | 1,020 | Hancock |
| Continental Structural Plastics | Automotive | 966 | NW Ohio |
| Block Communications, Inc. | Information Services | 955 | Lucas |
| NSG Pilkington | Advanced Manufacturing | 940 | NW Ohio |
| Lima Memorial Health System | BioHealth | 910 | Allen |
| Hollywood Casino | Retail | 892 | Lucas |
| Adient/Johnson Controls | Automotive | 881 | NW Ohio |
| Wood County Hospital | BioHealth | 875 | Wood |
| Cooper Farms | Food Sciences & Agriculture | 850 | NW Ohio |
| Owens-Illinois, Inc. | Advanced Manufacturing | 843 | Wood |
| Eaton | Advanced Manufacturing | 840 | Van Wert |
| FedEx Ground | Logistics & Distribution | 810 | Wood |
| Bridgestone APM Company | Advanced Manufacturing | 800 | Wyandot |
| First Solar | Advanced Manufacturing | 800 | Wood |
| MetoKote Corporation | Advanced Manufacturing | 800 | Allen |
| Lowe's Distribution Center | Logistics & Distribution | 750 | Hancock |
| Vehtek Systems, Inc. | Automotive | 750 | Wood |
| International Paper Company | Advanced Manufacturing | 730 | Hardin |
| Walgreens Distribution Center | Logistics & Distribution | 659 | Wood |
| Ohio Northern University | Higher Education | 653 | Hardin |
| Fulton County Health Center | BioHealth | 650 | Fulton |
| Kasai North America | Advanced Manufacturing | 650 | Wyandot |
| Materion Brush Inc. | Advanced Manufacturing | 635 | Ottawa |
| DTR Industries, Inc. | Advanced Manufacturing | 611 | Allen |
| Revere Plastics, Inc. | Advanced Manufacturing | 605 | Sandusky |
| Defiance Metal Products | Automotive | 602 | Defiance |
| BP Toledo Refining | Shale Energy & Petrochemicals | 600 | Lucas |
| Mobis North America LLC | Automotive | 600 | Lucas |
| Norfolk Southern Corp. | Logistics & Distribution | 600 | NW Ohio |
| Vaughn Industries | Other - Construction | 600 | Wyandot |
| Crown Battery Manufacturing | Advanced Manufacturing | 579 | Sandusky |
| Imasen Bucyrus Technology | Automotive | 562 | Crawford |
| Fifth Third Bank | Financial Services | 550 | NW Ohio |
| SpartanNash | Other - Retail | 550 | Allen |
| Kamco Industries Inc. | Automotive | 544 | Williams |
| Kalida Manufacturing, Inc. | Automotive | 542 | Putnam |
| University of Northwestern Ohio | Higher Education | 530 | Allen |
| Alex Products | Advanced Manufacturing | 519 | Henry |
| Kohl's Distribution Center | Logistics & Distribution | 508 | Hancock |
| Toledo Refining Company | Shale Energy & Petrochemicals | 500 | Lucas |
| National Machinery | Advanced Manufacturing | 493 | Seneca |
| ABC Inoac Exterior Systems | Automotive | 478 | Sandusky |
| Huntington National Bank | Financial Services | 471 | NW Ohio |
| Spangler Candy Company | Food Sciences & Agriculture | 460 | Williams |
| Ada Technologies, Inc. | Automotive | 455 | Hardin |
| Sanoh America | Automotive | 453 | Hancock |
| BEF Foods Inc | Food Sciences & Agriculture | 452 | Allen |
| KeyBank | Financial Services | 450 | NW Ohio |
| Rudolph/Libbe Companies | Construction | 450 | Wood |
| Roppe Rubber Company | Advanced Manufacturing | 425 | Seneca |
| Pittsburgh Glass Works | Automotive | 424 | Crawford |
| Medical Mutual of Ohio | BioHealth | 423 | Lucas |
| ADP Company | Information Services | 421 | Hancock |
| Bellevue Hospital | BioHealth | 420 | Sandusky |
| Husky Energy Lima Refinery | Shale Energy & Petrochemicals | 420 | Allen |
| ROKI America Co., Ltd. | Automotive | 409 | Hancock |
| Ball Metal Beverage Packing | Advanced Manufacturing | 404 | Hancock |
| Range Kleen Manufacturing, Inc. | Advanced Manufacturing | 403 | Allen |
| Calphalon a division of Newell Rubbermaid | Advanced Manufacturing | 400 | Wood |
| Federal Mogul | Advanced Manufacturing | 400 | Van Wert |
| Kraft Heinz | Food Sciences & Agriculture | 378 | Sandusky |
| Central Insurance | Financial Services | 369 | Van Wert |
| North Star / BlueScope Steel | Advanced Manufacturing | 365 | Fulton |
| KUKA Toledo Production Operations | Automotive | 355 | Lucas |
| Autoneum North America, Inc. | Automotive | 350 | Lucas |
| CSX Intermodal | Logistics & Distribution | 350 | NW Ohio |
| Gerken Construction Company | Construction | 350 | Henry |
| Progressive Stamping, Inc. | Automotive | 350 | Putnam |
| Wyandot Memorial Hospital | BioHealth | 350 | Wyandot |
| SSOE Group | Information Services | 349 | Lucas |
| CK Technologies | Automotive | 340 | Williams |
| Electroprime | Automotive | 340 | Wood |
| Matsu Ohio | Automotive | 328 | Williams |
| Bucyrus Precision Tech, Inc | Automotive | 326 | Crawford |
| Heidelberg University | Higher Education | 325 | Seneca |
| Ohio Health Hardin Memorial Hospital | BioHealth | 325 | Hardin |
| Power & Sons LLC. | Automotive | 322 | Williams |
| Chase Brass & Copper Co LLC. | Advanced Manufacturing | 320 | Williams |
| Style Crest Products | Advanced Manufacturing | 314 | Sandusky |
| Pro-Tec Coating Company | Advanced Manufacturing | 310 | Putnam |
| Allied Moulded Products | Advanced Manufacturing | 305 | Williams |
| Best Buy Distribution | Logistics & Distribution | 300 | Hancock |
| Custom Glass Solutions by Guardian | Advanced Manufacturing | 300 | Wyandot |
| Kuss Corporation | Advanced Manufacturing | 300 | Hancock |
| Lakeview Farms, Inc | Advanced Manufacturing | 300 | Allen |
| Namsa | Information Services | 300 | Wood |
| PT Services Rehabilitation | BioHealth | 300 | Seneca |
| Unverferth Manufacturing Company, Inc. | Advanced Manufacturing | 296 | Putnam |
| G.S.W. Manufacturing, Inc. | Automotive | 288 | Hancock |
| Ameriwood Industries | Advanced Manufacturing | 285 | Seneca |
| Henry County Hospital | BioHealth | 285 | Henry |
| Cooper Standard Automotive | Automotive | 281 | Wood |
| GE Lighting-Bucyrus Lamp Plant | Advanced Manufacturing | 276 | Crawford |
| Webster Industries | Advanced Manufacturing | 275 | Seneca |
| Timken Company | Advanced Manufacturing | 274 | Crawford |
| Bemis Company, Inc. (formerly Curwood) | Advanced Manufacturing | 273 | Sandusky |
| Braun | Automotive | 270 | Van Wert |
| Covert Manufacturing | Advanced Manufacturing | 262 | Crawford |
| Hylant | Information Services | 255 | Lucas |
| J.M. Smucker | Food Sciences & Agriculture | 250 | Lucas |
| Kalmbach Feeds Inc. | Food Sciences & Agriculture | 250 | Wyandot |
| Proctor & Gamble Manufacturing Company | Advanced Manufacturing | 250 | Allen |
| St. Francis Senior Ministries | BioHealth | 250 | Seneca |
| Altenloh, Brinck & Co. (ABC US) | Advanced Manufacturing | 240 | Williams |
| ConAgra Foods | Food Sciences & Agriculture | 235 | Fulton |
| ZF TRW | Automotive Manufacturing | 235 | Fulton |
| Graham Packaging Plastic Products | Advanced Manufacturing | 234 | Hancock |
| Production Products, Inc. | Automotive | 234 | Putnam |
| Tower Automotive | Automotive | 234 | Sandusky |
| Ohio Mutual Insurance Group | Financial Services | 233 | Crawford |
| United States Gypsum Co. | Advanced Manufacturing | 233 | Ottawa |
| Accubuilt | Advanced Manufacturing | 230 | Allen |
| Concordance Healthcare Solutions (formerly Seneca Medical) | BioHealth | 230 | Seneca |
| Faurecia Emissions Control | Automotive | 230 | Lucas |
| DS Brown | Advanced Manufacturing | 228 | Wood |
| Principle Business Enterprises | Advanced Manufacturing | 221 | Wood |
| Excel | Automotive | 220 | Lucas |
| Tiffin Developmental Center | BioHealth | 217 | Seneca |
| Tiffin University | Higher Education | 217 | Seneca |
| Church & Dwight | Advanced Manufacturing | 216 | Seneca |
| Good Shepherd Home | BioHealth | 215 | Seneca |
| Old Castle Building Envelope | Advanced Manufacturing | 215 | Wood |
| Advanced Drainage System | Advanced Manufacturing | 212 | Hancock |
| Atlas Industries, Inc. | Automotive | 206 | Sandusky |
| Precision Automotive Plastics | Automotive | 202 | Sandusky |
| Autoplas (Windsor Mold) | Automotive | 200 | Sandusky |
| Betco Corporation | Advanced Manufacturing | 200 | Wood |
| Evergreen Plastics/Division of Polychem | Advanced Manufacturing | 200 | Sandusky |
| Farmers & Merchants State Bank | Financial Services | 200 | NW Ohio |
| Haas Door Company | Manufacturing | 200 | Fulton |
| Tenneco | Automotive | 200 | Henry |
| Wilbert Plastics | Automotive | 200 | Sandusky |
